PaeR7I does not recognize the sequence CTCTCGAG. Prolonged incubation with NdeI may lead to removal of additional nucleotides.Īfter cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.ĮcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage. Sticky ends from different Bpu10I sites may not be compatible.
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